At the Gate

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Can I get an even better deal at the gate?

Yes, check-in can provide an opportunity for a cheap or possibly even free upgrade into a better class. If one of the better classes of seats has room, you might be able to get a real good upgrade deal. Some Airlines wait until boarding before they start the dealing, but ask at check-in. You can find out whom to talk to and you might just get lucky. Here are a few tips to help you into that better seat.
Being in their elite member or frequent flyer clubs will help.
Producing your membership in another Airline's elite club and saying "I wanted to give you guys a chance to win my business" could win you that first class seat.
Dressing for success. Do you look like you should be in first class?
If you are real nice and friendly, you might even be able to get a free upgrade.

Can an Airline refuse to board me if I have a valid ticket?

Yes, you can be refused boarding for many reasons. Here are a few:

Failing to present proper I.D., passport or other necessary governmental paperwork to complete your trip.
The name on your ticket doesn't match the name on your I.D.
Refusing to allow a search of your luggage or your person.
Behaving in an abusive, disorderly or violent manner.
Being under the influence of drugs or alcohol.
Refusing to cooperate with any reasonable request made by security or Airline personnel.